Dupret Group

dupret

Firing response of hippocampal CA1 principal cells during sharp-wave/ripple events in sleep. Top trace, wide-band (1H–5 kHz) local field potential recorded in the pyramidal cell layer. Bottom trace, 140–250 Hz band pass-filtered local field potential highlighting ripple frequency events. Raster plots, spike times of simultaneously recorded CA1 principal cells (one cell per row). Note the firing synchrony during ripple events.

The objective of our programme is to define how coordination of neuronal activity in the hippocampus and connected subcortical circuits contributes to memory processes during active waking behaviour and sleep.

Group Science

The group performs circuit analyses to: (i) determine how neural representations of the external world are computed, consolidated and recalled in the hippocampus for the purpose of memory;  (ii) establish the mnemonic contribution of oscillatory patterns of network activity (e.g., theta, gamma, sharp wave/ripples); and (iii) define neuronal interactions across brain circuits during the expression of memory-guided behaviours.

This research requires multi-channel extracellular recordings to monitor the parallel activity of multiple neurons together with network oscillations as measured by fluctuations in local field potentials. Principal cells and interneuron types are discriminated based on criteria that have been previously established using dual intracellular/extracellular or juxtacellular recordings in acute in vivo recordings. In order to draw causal relationships between neuronal activity and behaviour, we combine such recordings with optogenetic techniques to manipulate specific sets of neurons, stimulating or silencing them, and determine their contributions at a high spatio-temporal resolution.

Key Research Areas
  • In vivo study of neuronal ensembles during behaviour
  • Mnemonic contribution of network oscillations
Research Techniques
  • Neuronal ensemble recording and associated data analyses
  • Optogenetics
  • Behavioural analyses

Recent Publications

Unit Publication
Joshi A
Salib M
Viney TJ
Dupret D
Somogyi P

2017.Neuron, 96(6):1342-1357.e5.

Unit Publication
van de Ven GM
Trouche S
McNamara CG
Allen K
Dupret D
2016.Neuron, 92(5):968-974.
Unit Publication
Trouche S
Perestenko PV
van de Ven GM
Bratley CT
McNamara CG
Campo-Urriza N
Black SL
Reijmers LG
Dupret D

2016.Nat. Neurosci., 19(4):564-7.

McNamara CG
Tejero-Cantero Á
Trouche S
Campo-Urriza N
Dupret D
2014.Nat. Neurosci., 17(12):1658-60.
Dupret D
Csicsvari J
2014. Nat. Neurosci., 17(5): 643-4.