Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission.

Raimondo JV
Kay L
Ellender TJ
Akerman CJ
Scientific Abstract

Optogenetic silencing using light-driven ion fluxes permits rapid and effective inhibition of neural activity. Using rodent hippocampal neurons, we found that silencing activity with a chloride pump can increase the probability of synaptically evoked spiking after photoactivation; this did not occur with a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.

Citation

2012.Nat. Neurosci., 15(8):1102-4.

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