Dr. Kouichi C. Nakamura

Dr. Kouichi C. Nakamura

Senior Postdoctoral Neuroscientist

Dr. Kouichi Nakamura graduated in biological sciences at Kyoto University, Japan, in 2000. He then studied postnatal development of glutamatergic neuronal circuits under the supervision of Professor Takeshi Kaneko, and obtained his Ph.D. from the Graduate School of Medicine at Kyoto University in 2006. Dr. Nakamura then stayed in the Kaneko laboratory as a postdoctoral researcher to extend his studies on the postnatal development of the basal ganglia circuits. Dr. Nakamura joined the Magill Group as a Long-Term Fellow of the Human Frontier Science Program Organization in September 2009. After the completion of his Fellowship in September 2012, he continued his studies of the motor thalamus in the Unit as an MRC Investigator Scientist. Dr. Nakamura returned to Kyoto University in September 2013 to serve as an Assistant Professor.

In May 2016, Dr. Nakamura rejoined the Magill Group as a Senior Postdoctoral Neuroscientist in order to pursue his long-standing research interest on the structure and function of the motor thalamus in health and disease.

In vivo electrophysiological recording techniques, anatomical methods (including immunohistochemistry, fluorescence in situ hybridization histochemistry, and confocal microscopy), and the use of genetics-based approaches for the monitoring and manipulation of specified cell types, are all central to Dr. Nakamura’s research strategy to elucidate the principles governing neuronal communication within the basal ganglia and partner thalamic circuits.

Key Research Areas

Processing of movement-related information by neurons in the thalamus, basal ganglia, cerebral cortex and cerebellum.
Behavioural correlates of neuronal information processing.
Experimental models of Parkinson's disease.
Develpment of CHAMBER (CHemoarchitectonic Atlas of the Mouse thalamus as a BNDU OpEn Resource) to provide publicly available anatomical reference for the delineation of brain structures, such as thalamic nuclei.

kouichi.nakamura@bndu.ox.ac.uk

Head of Group

Prof. Peter J. Magill

Parasagittal section of the rat neostriatum at postnatal day 4. The tissue was triple-labelled for tyrosine hydroxylase (TH, green), vesicular glutamate transporter 1 (VGluT1, blue), and VGluT2 (red), markers for the three major afferents to neostriatum. 'Dopamine islands', the areas of intense TH immunoreactivity, often overlap with 'glutamate islands', the areas of intense VGluT1 and VGluT2 immunoreactivities. The three major afferents thus together form 'afferent islands' in the neostriatum of rat pups. For further details, please see Nakamura et al. (2005, 2009).

A parasagittal section (lateral ∼1.5 mm) of the rat thalamus fluorescently labeled with immunoreactivities for GAD67 (green),VGluT2 (red), and VGluT1 (blue) shows GAD67-rich basal ganglia-recipient zone and VGluT2-abundant cerebellar recipient zone of the motor thalamus. See Nakamura et al. 2014. Temporal coupling with cortex distinguishes spontaneous neuronal activities in identified basal ganglia-recipient and cerebellar-recipient zones of motor thalamus. Cereb Cortex 24(1): 93–109.

This image won the second prize in BRAINScapes Image Competition 2019 in Oxford Neuroscience Symposium on 20 Mar 2019.

Title: A Vivid Look of the Mouse Brain

Lay description:

Here we show a slice of the mouse brain in a riot of colours. Different colours are used to detect special proteins and nucleic acid (Nissl staining) to highlight the diversity in brain structure at great detail. How many structures can you recognize?

Scientific description:

This is an image of a parasagittal section of the mouse brain fluorescently labelled for anti-calretinin (green), anti-calbindin (red), and anti-parvalbumin (blue) as well as Nissl staining (white). Nissl staining has a long history of being used for studying the cytoarchitecture of the brain and considered a basis of neuroanatomy. Calretinin, calbindin, and parvalbumin are all calcium-binding proteins and specific antibodies against them have been used to visualize the chemoarchitecture of the brain and used to delineate structures that are difficult to be identified on the basis of cytoarchitecture alone. In this sub-micron resolution image (with more than 300 million pixels by 4 channels; 0.641 µm in tissue per pixel), you can recognize individual cell bodies and even some dendrites. This is a subset of the data deposited on our CHAMBER website (CHemoarchitectonic Atlas of the Mouse thalamus as a BNDU opEn Resource), which are now publicly available to facilitate precise delineation of brain structures (https://data.mrc.ox.ac.uk/chamber).